Estimating Freezing Injury on Olive Trees: A Comparative Study of Computing Models Based on Electrolyte Leakage and Tetrazolium Tests

Winter frost injury is a major limiting factor for olive cultivation in temperate regions. The response of shoots to freezing stress can be used selecting genotypes resistant freezing. electrolyte leakage (EL) and tetrazolium tests (TZ) are commonly evaluate dead tissues cold studies. temperature–response curve lethal temperature (LT) measured with models calculate LT50 LT90. In this study, we evaluated the accuracy efficiency eighteen nonlinear regression (NLRs) calculating LT90 different cultivars at various stages dormancy. After evaluating prediction performance NLR models, it was found that only eight were suitable purpose research out examined. 2p-logistic Gompertz selected modeling EL TZ, respectively. Our findings indicate Roughani, Kawi, Zard varieties trees exhibit best under artificial temperature-controlled conditions. provide valuable insights into frost-resistant designing effective strategies acclimation cultivation.